Measurement

Part:BBa_J100430:Design

Designed by: Tatianna Travieso   Group: Campbell M Lab   (2018-06-28)


T7 Promoter with Downstream Gal4 Binding Site in pClone mScarlet


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 912
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 912
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 912
    Illegal BamHI site found at 858
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 912
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 912
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ikeda's paper referenced earlier discussed studies that found an "initiation domain" and a "binding domain" from -12 to +5, both of which were essential for successful transcription and translation. It also mentions a "dispensable region" from -12 to -17 that is able to be deleted or changed.


Source

"T7 promoter essential for promoter activity in vivo" by Richard A. Ikeda et al. (1992), https://www.ncbi.nlm.nih.gov/pubmed/1598210 Consensus sequence, https://parts.igem.org/Promoters/Catalog/T7Promoters/Catalog/T7 "Structure and function of Zn(II) binding site within the DNA binding domain of the Gal4 transcription factor" by Tao Pan et al. (1989), https://www.ncbi.nlm.nih.gov/pubmed/2497463


References